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We talk about nutrients, food, HACCP, biotouch and PMEU in utilizing and studying microbes in industry or medicine or in environmental cleaning, the used microbiological and bacteriological methods in most cases call for the culturing of cells or equivalent...

Website: http://biotechtouch.blogspot.com/
Location: Sotkamo, Jyväskylä, Kuopio, Mexico City
Members: 7
Latest Activity: Jul 6, 2014

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Vesi ja Visko - Bakteerit ja Mikrobit

Started by Helge V. Keitel. Last reply by Helge V. Keitel Nov 15, 2009. 2 Replies

GMO Food and the world's wood supply

Started by Helge V. Keitel. Last reply by Helge V. Keitel Nov 16, 2008. 1 Reply

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Comment by KK-Net on January 23, 2014 at 9:55pm

Olemme siirtyneet jälkiruokaan.

Comment by KK-Net on January 12, 2014 at 12:29am

Muutama kuukausi grillikauden alkuun.

Comment by Helge V. Keitel on November 15, 2009 at 10:10am
Juha, nice to see you back here. You visited China recently, was that about the "fastest method in the world to detect microbial growth?"
Comment by Juha Veikko Mentu on November 15, 2009 at 10:05am
The R&D activities of Samplion Ltd. and Finnoflag Ltd. have now led to two innovative models of PMEU: model Spectrion measures the turbidity of test syringes, caused by the microbial growth, and another model called Scentrion collects microbial metabolites from the syringes to a gas analyzator. The last one is, as far as I know, the fastest method in the world to detect microbial growth - a real "Rapid Method".

More infromation is available in www.rapidmicrobiologywithpmeu.blogspot.com
and www.samplion.fi.
Comment by Juha Veikko Mentu on June 14, 2009 at 2:46pm
I will deliver the latest post by my closest friend, IM, for the readers of Biotouch Water Enviro. It mainly concerns the microbiology of paper industry but shows also some challenges of PMEU methods, however:

Certain types of microbiological problems in paper mills seem to be acute all the time. Looking back to last months, this may be "Top Three" among them:

* Microbiological spoilage of raw materials. This is an everlasting hazard for mineral and starch slurries, and the reasons are very easy to understand: both raw materials mentioned may contain high densities of bacteria (mainly aerobic sporeformers and actinobacteria), slurries containing starch are very nutritive growth media for different microbial species and the very challenging biocidic treatments of slurries (especially mineral ones), when inaccurate, can lead to the total spoilage of them.

* Growth of biofilm and production of slime. This problem seems to be connected to poor washing and boil-out programs which leave rests of biofilm inside the machine and give growth time for it because too long running periods. The chose of ineffective biocide and/or its insufficient dosing can also stimulate the activity of these trouble-makers.

* Microbial growth in the broke system. Especially big machines with large broke towers suffer of this problem. If the basic biocide program is insufficient and the retention times inside the towers are too long, aerobic population tend to increase the number of its cells to the level of 10 000 000 cfu/g or even higher. Consumption of oxygen by respirating bacteria leads to anaerobic conditions, redox potential will be dropped and the growth conditions for both fermentative and anaerobic bacteria turns to be excellent. Drop of pH, slime and spore formation, smells and odours - even the production of H2S and H2 - will be found in such situations.

There are some measures to prevent these hazards. Growth period of microbial population shall be kept as short as possible, the control of waterborne and bioflim bacteria shall be as rapid and frequent as possible and the bioside programs, intended in killing of raw material, process water and biofilm bacteria shall be evaluated more frequently.

A realistic and accurate way to control both process water and biofilm growth, as well as to evaluate biocide programs, is now available. The Finnish company SAMPLION Ltd is manufacturing and selling "Portable Microbiological Enrichment Unit", a "mini-fermentor" for 10 simultaneous tests in controlled conditions, to detect the failures of biocide programs in only hours (watesr) or days (biofilms). Some results of PMEU's paper industry applications will be published in next Spring - coming back to refer them later.

Some wrong ideas about the overdosing of biocides will also rise up frequently among publicity. Basically it is not a question of only the cubic meters of biocides consumed, however, The chose of most effective biocides for different areas of processes towards different problems, the dosing of these compounds, their type of action and some other factors play a major role when building an effective biocide program for paper machine. Overdosing of biocides is a problem only in cases, when the program does not work, and leads to the loss of money and the rise of biocide concentration in paper machine effluents.
Comment by Helge V. Keitel on February 28, 2009 at 10:41am
I've a contact from Canada about biotechnology blogging and cooperation. We could chat about that some day over Skype.
Comment by Juha Veikko Mentu on February 27, 2009 at 4:30pm
Lähetän luettavaksenne Industrymicrobiologistin jutun. Paperiteollisuuden jätevedenpuhdistus kärsii ainakin paikoitellen vakavista ongelmista, joihin voisi paneutua tarkemmin mm. PMEU-menetelmää soveltaen.

Here is the latest post by Industrymicrobiologist:

Effluents of pulp and paper mills are relatively simple to treat by activated sludge process - in theory. Some severe problems have arisen in practice, however.

Temperatures of incoming waste waters have risen during last years. The most obvious reason for this phenomenon is the increased recirculation of process waters inside the mills. Poor sedimentation of flogs, caused by the heavy growth of filamentous bacteria, has also become to be more and more hard problem for purification plants. There may be some kind of correlation between temperature and bulking of sludge?

Another problem, potentially very hazardous, is the growth and recirculation of Legionella pneumophila inside activated sludge process. The role of Protozoa has now been established: they act as carrirs of bacteria backwards in returned sludge.

Dosing of nutrients into biological treatment steps isn't any hard task anymore because the need of phosphorus and nitrogen has been studied very deeply, and simpe AT LINE testing for the optimization of them is easy to perform in the mill labs.

Two problems, mentioned before, still exist and need R&D activities to be solved: bulking of the sludge (by filamentous organisms) and L.pneumophila.
Comment by Helge V. Keitel on November 27, 2008 at 2:48pm
@totoriki kirjoitti Jaikussa äsken Sitran Food konferenssista: Strategisella ketteryydella kolme ajuria: avoin strategiaprosessi, noussut strateginen hälytysvalmius, laadukas sisäinen keskustelu
Comment by KK-Net on November 24, 2008 at 12:59pm
Take a look at the BIONEERS COMMUNITY.
Comment by Helge V. Keitel on November 18, 2008 at 9:25pm
Juha, 1991 - 2008 ei ole tapahtunut suuria muutoksia.

"Uuden biotekniikan sovellutuksissa tutkimus- ja tuotekehitysvaihe on yleisesti ollut jopa yli kymmenen vuoden mittainen. Vaikka tuote on ollut jo kauan teknisesti valmis, tiukat turvallisuusvaatimukset ja selkeiden pelisääntöjen puuttuminen hyväksyttämisessä ovat usein viivästyttäneet pääsyä markkinoille."

Tuo on edelleen täysin totta. Uskon kuitenkin, että tämä meidän parveilumme ja joukkovoimamme huomataan ja HUPS HEIJAA yhtäkkiä jutut lähtevät luistamaan. Vosin kirjoitta aiheesta teorian vaikkapa tämän päivän kokemuksista (seminaari), mutta palataan siihen.

Vastaukseni löytyy PPI kommentista. Emme ole kaukana, sano minun sanoneen.
 

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